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KMID : 0648420110160010024
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Journal of Korean Society of Pediatric Endocrinology
2011 Volume.16 No. 1 p.24 ~ p.30
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The Effect of IGFBP-3 on Adipokines and Gene Expression in Differentiated 3T3-L1 Adipocytes
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Kim Hae-Soon
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Abstract
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Purpose: IGFBP-3 leads to the induction of insulin resistance in 3T3-L1 adipocytes. We carried out a series of
experiments to elucidate the effects of IGFBP-3 on adipokines and gene expressions.
Methods: We treated fully-differentiated 3T3-L1 adipocytes with IGFBP-3 (0.5, 1, and 2 μg/mL) for one day and
measured the mRNA levels of adiponectin, leptin, resistin, and TNF-α by RT-PCR, and adiponectin, leptin, resistin,
and IL-6 protein levels in the culture supernatant were measured using multiplex adipokine assay ELISA Kits (Linco
Research, St. Charles, Missouri). Gene expression in 3T3-L1 adipocyte cells using a microarray method was performed.
Results: IGFBP-3 inhibited the expression of adiponectin, leptin, resistin, and TNF-α mRNA. IGFBP-3 at 0.5 and
1 μ/mL decreased adiponectin release, but IL-6 release was increased at 2 μ/mL IGFBP-3. A dose-dependent
inhibition of leptin was released by IGFBP-3 at 50%. Resistin release was decreased by 40%. The effect of IGFBP-3
on the gene expression in 3T3-L1 adipocyte cells using a microarray assay related to an increase of agouti-realted
proteins (Agrp) and Janus kinase 2 (JAK2), and a decrease of the ras homolog gene family (Rhoq), acyl-CoA synthetase
long-chain family member 6 (Acsl6), and the interleukin-1 receptor-associated kinase 1 (Irak1).
Conclusion: IGFBP-3 regulates several adipokines gene expressions that are known to modulate insulin sensitivity,
and this regulation may be attributable to the insulin resistance effect of IGFBP-3 on adipocytes.
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KEYWORD
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Insulin-like growth factor binding protein 3, Insulin resistance, Adipokines
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